Methods and compositions for concentrating secreted recombinant protein
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United States of America Patent
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Jul 16, 2013
Grant Date -
May 3, 2012
app pub date -
Jan 9, 2012
filing date -
Feb 28, 2002
priority date (Note) -
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Abstract
Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.
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