Method and device for detecting the presence of a single target nucleic acid in a sample

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United States of America Patent

PATENT NO 8563275
APP PUB NO 20120301884A1
SERIAL NO

13572649

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Abstract

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A method comprising subjecting one or more sample portion(s) to a single amplification step, thereby amplifying a single molecule in the sample portion to a detectable level, and, in some embodiments, then determining whether the sample portion contains at least one molecule of the target nucleic acid. In some embodiments, the sample portion is in a porous sample structure, or in a sample chamber which comprises means for minimizing diffusion of the sample portion, or in a sample chamber which is inside a microcapillary device, or in a sample retaining means.

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Patent Owner(s)

  • APPLIED BIOSYSTEMS, LLC;THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Brown, James F Clifton, US 60 5040
Silver, Jonathan E Bethesda, US 22 3066

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