Nucleic acid amplification in the presence of modified randomers

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United States of America Patent

PATENT NO 8592184
APP PUB NO 20130109060A1
SERIAL NO

13711846

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Abstract

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The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.

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Patent Owner(s)

  • ROCHE MOLECULAR SYSTEMS, INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ankenbauer, Waltraud Penzberg, DE 29 425
Heindl, Dieter Paehl, DE 138 1124
Laue, Frank Paehl-Fischen, DE 16 75

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