Lysis and reverse transcription for MRNA quantification

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United States of America Patent

PATENT NO 8623602
APP PUB NO 20110111463A1
SERIAL NO

13008949

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Abstract

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The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol.

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Patent Owner(s)

  • ROCHE MOLECULAR SYSTEMS, INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kubista, Michael Moelndal, SE 6 22
Stroembom, Linda Goeteborg, SE 5 22
Zoric, Neven Goeteborg, SE 3 2

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