cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract

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United States of America Patent

PATENT NO 8623626
APP PUB NO 20110136204A1
SERIAL NO

12912724

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Abstract

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It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.

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Patent Owner(s)

Patent OwnerAddress
NATIONAL FOOD RESEARCH INSTITUTETSUKUBA-SHI IBARAKI 305-8642

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chumpolkulwong, Namtip Yokohama, JP 4 0
Hori, Chie Yokohama, JP 2 0
Hosaka, Takeshi Tsukuba, JP 6 32
Kigawa, Takanori Yokohama, JP 14 16
Ochi, Kozo Tsukuba, JP 6 61
Shirouzu, Mikako Yokohama, JP 18 36
Yokoyama, Shigeyuki Yokohama, JP 81 779

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