Methods and systems for collecting cells of a biological specimen
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United States of America Patent
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Mar 11, 2014
Grant Date -
Mar 17, 2011
app pub date -
Sep 11, 2009
filing date -
Sep 11, 2009
priority date (Note) -
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Abstract
Methods and systems for collecting cells on a filter disposed in a specimen fluid containing suspended cells of a biological specimen. A short vacuum pulse is applied across a filter to sip specimen fluid. A percentage or portion of the filter surface area covered by cells is determined is representative of or correlates to the density of cells in the specimen fluid. A maximum vacuum amplitude and/or duration are determined utilizing the determined filter coverage. A longer, continuous vacuum or slurp is applied across the filter to collect cells on the filter while limiting the amplitude and/or duration of the slurp based at least in part upon the determined maximum vacuum amplitude and/or duration.
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