Artifact apparatus to mimic reflection losses of solution-filled microtiter plate readers and related uses thereof
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United States of America Patent
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Apr 8, 2014
Grant Date -
Feb 14, 2013
app pub date -
Aug 9, 2011
filing date -
Aug 9, 2011
priority date (Note) -
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Abstract
An apparatus used to calibrate microtiter plate readers. The apparatus includes one or more structures having two exterior surfaces wherein one of the exterior surfaces is coated with a coating selected to establish on that surface a reflection loss that mimics reflection loss at an air-liquid interface, and the other of the two exterior surfaces is coated with a coating selected to establish a reflection loss on that surface that mimics the reflection loss at a bottom surface of a microtiter plate. The apparatus may be a single layer, a multi-layered composition or a container. The apparatus is an artifact that may be used to calibrate a plate reader by mimicking a solution-filled microtiter plate. The artifact may be used for a plurality of liquids, including water. The artifact produces reflection losses more closely mirroring reflection losses expected for a liquid-filled microtiter plate well.
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