Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest
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United States of America Patent
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Apr 22, 2014
Grant Date -
Nov 8, 2012
app pub date -
Jun 21, 2012
filing date -
Jun 24, 2003
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Abstract
The invention is directed to enhanced methods for detecting an analyte of interest in situ, by immunoassay, or by hybridization comprising binding an enzyme-labeled conjugate molecule to an analyte of interest in the presence of a redox-inactive reductive species and a soluble metal ion. The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition.
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