Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences
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United States of America Patent
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Jun 10, 2014
Grant Date -
Mar 5, 2009
app pub date -
Aug 30, 2007
filing date -
Aug 30, 2007
priority date (Note) -
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Abstract
Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.
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