Isolation and characterization of novel green fluorescent proteins from copepods
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United States of America Patent
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Jul 8, 2014
Grant Date -
Mar 22, 2012
app pub date -
Mar 10, 2010
filing date -
Mar 20, 2009
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Abstract
The isolation and characterization of two protein isoforms collected green fluorescent copepods is described herein. The new Pontella mimocerami GFP-like isoforms pmimGFP1 and pmimGFP2 of the present invention are quick to mature and rapidly produce a fluorescent signal. The two isoforms are very similar in molar extinction coefficients (ME) with 105,000 M−1 cm−1 for pmimGFP1 and 103,000 M−1cm−1 for pmimGFP2, respectively. The relative brightness of these two new copepod GFP-like proteins is the highest measured for any isolated GFP-like protein.
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