Expression vectors based on modified ribosomal protein promoters and uses thereof in post-transcriptional assessment
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United States of America Patent
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Aug 5, 2014
Grant Date -
Dec 22, 2011
app pub date -
Nov 17, 2008
filing date -
Jun 27, 2008
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Abstract
The present invention relates to expression vector comprising (a) a promoter region comprising a non-inducible constitutively active ribosomal protein gene promoter, (b) an operably linked reporter or gene sequence, and (c) a 3′ untranslated region (3′ UTR), which are suitable means for an selective assessment of post-transcriptional regulation, post-transcriptional control elements and factors as well as for identifying compounds that effect post-transcription. The present invention furthermore relates to arrays, expression vector libraries and cell lines containing the expression vector(s). The present invention furthermore relates to a method and kit for identifying compounds that affect post-transcriptional regulation of reporter(s) or gene(s), that utilize the expression vector(s).
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