Device for amplifying target nucleic acid
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United States of America Patent
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Sep 2, 2014
Grant Date -
Jun 6, 2013
app pub date -
Feb 12, 2013
filing date -
Apr 17, 1997
priority date (Note) -
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Abstract
A device for amplifying target nucleic acid in a sample can include a planar fluidic assembly including a transparent substrate, a porous material layer on a surface of the transparent substrate, and a cover over the porous material layer and sealingly affixed to the substrate. The cover may be spaced from the porous material layer and a flow channel defined between the porous material layer and the cover. The flow channel may have a uniform cross-section from a first end to a second end. The assembly can further include an inlet in flow communication with the first end of the flow channel to introduce sample containing target nucleic acid into the flow channel, an outlet in flow communication with the second end of the flow channel, and a plurality of nucleic acid primers retained by the porous material layer at discrete regions along and within the flow channel, each of the plurality of nucleic acid primers being complementary to a portion of the target nucleic acid in the sample to enable a primer-based amplification reaction of the target nucleic acid. The porous material layer may be configured to retain, at the discrete regions and during a primer-based amplification reaction, sample introduced to the flow channel and amplified product of the amplification reaction.
First Claim
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- 15 United States
- 10 France
- 8 Japan
- 7 China
- 5 Korea
- 2 Other
Patent Owner(s)
- APPLIED BIOSYSTEMS, LLC;THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES;THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND SCIENCES
International Classification(s)
- [Classification Symbol]
- [Patents Count]
Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
Brown, James F | Clearwater Beach, US | 60 | 5040 |
Silver, Jonathan E | Bethesda, US | 22 | 3066 |
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