Method to improve the stability and broaden the pH range of family G/11 xylanases
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United States of America Patent
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Sep 30, 2014
Grant Date -
Oct 31, 2013
app pub date -
Mar 22, 2013
filing date -
Oct 12, 1999
priority date (Note) -
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Abstract
The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
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