Method for detecting the presence of a target nucleic acid sequence in a sample
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United States of America Patent
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Oct 14, 2014
Grant Date -
Feb 5, 2009
app pub date -
Aug 13, 2007
filing date -
Apr 17, 1997
priority date (Note) -
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Abstract
A method comprises loading a sample portion into a sample chamber which comprises means for minimizing diffusion of the sample portion, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a sample portion and a sample chamber comprising means for minimizing diffusion of the sample portion. Also, a microfluidic device comprising a sample chamber and an amplification targeting reagent positioned in the first sample chamber.
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