-glycans in lower eukaryotes

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United States of America Patent

PATENT NO 8883483
SERIAL NO

13156804

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Abstract

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The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

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Patent Owner(s)

Patent OwnerAddress
GLYCOFI INCLEBANON NH

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bobrowicz, Piotr Hanover, US 49 760
Choi, Byung-kwon Hanover, US 62 729
Davidson, Robert Hanover, US 75 677
Gerngross, Tillman U Hanover, US 49 1421
Hamilton, Stephen Lebanon, US 24 242
Nett, Juergen Enfield, US 14 78
Wildt, Stefan Lebanon, US 43 1006

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