Minicircle DNA vector preparations and methods of making and using the same
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United States of America Patent
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Feb 3, 2015
Grant Date -
Feb 7, 2013
app pub date -
Aug 6, 2012
filing date -
Jul 3, 2008
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Abstract
The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications.
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