Hot start reverse transcription by primer design

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United States of America Patent

PATENT NO 8993240
APP PUB NO 20100317062A1
SERIAL NO

12504633

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Abstract

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The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.

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Patent Owner(s)

  • APPLIED BIOSYSTEMS, LLC

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Lao, Kai Qin Pleasanton, US 72 992
Livak, Kenneth J San Jose, US 78 3061
Straus, Neil A Emeryville, US 32 448

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