Microscopy system and method for creating three dimensional images using probe molecules
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United States of America Patent
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Mar 31, 2015
Grant Date -
Jun 13, 2013
app pub date -
Mar 18, 2010
filing date -
Mar 18, 2009
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Abstract
A system (100) and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage (160). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera (155). Object planes in corresponding recorded regions of interest are recorded in the camera (155). A signal from the regions of interest is combined into a three dimensional image.
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