Method for identification and purification of multi-specific polypeptides
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United States of America Patent
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Apr 28, 2015
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Feb 13, 2014
app pub date -
Mar 15, 2012
filing date -
Mar 17, 2011
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Abstract
The document pertains to a method for the purification of a ternary mixture of dimeric antibodies of the type AA, AB, BB, characterized in that for the separation of the three components and in particular for the isolation of the multi-specific fraction AB multicolumn counter current solvent gradient purification chromatography with a stationary phase load of more than 1 mg antibody mixture per milliliter stationary phase is used. It furthermore relates to a method for the identification of in particular bispecific antibody systems, which are particularly suitable for the application of such a purification method.
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