Method for immune cell-assisted bacterial culturing
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United States of America Patent
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Jun 9, 2015
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Feb 27, 2014
app pub date -
Aug 21, 2013
filing date -
Aug 21, 2012
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Abstract
A method of culturing fastidious bacteria where a conditioned cell culture medium is prepared in which eukaryotic cells have been cultured, but where the medium is substantially free of the cultured cells. Fastidious bacteria are cultured in the medium. The conditioned cell culture medium can contain secreted factors from the cells. These factors promote the growth of the fastidious bacteria. The fastidious bacterial culture is maintained for a time period sufficient for the fastidious bacteria to multiply. The fastidious bacteria are analyzed using PCR, DNA sequencing or microbiology characterization.
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