Detection and quantification of biomolecules using mass spectrometry
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United States of America Patent
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Jun 9, 2015
Grant Date -
Aug 21, 2014
app pub date -
Feb 13, 2013
filing date -
Dec 5, 2006
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Abstract
The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
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