Recombinase polymerase amplification reagents and kits
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United States of America Patent
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Jun 16, 2015
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app pub date -
Jun 7, 2010
filing date -
Jun 5, 2009
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Abstract
This disclosure describes kits, reagents and methods for Recombinase Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed kits, reagents and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
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