Methods for measuring analyte transport across barriers using x-ray fluorescence
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United States of America Patent
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Jun 23, 2015
Grant Date -
Sep 12, 2013
app pub date -
Apr 26, 2013
filing date -
Jul 1, 2008
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Abstract
The present invention comprises a method for measuring the transport of analytes through a cell. The method comprises the steps of providing one or more cells containing at least 10 picograms of an analyte within a volume defined by the area of an x-ray excitation beam and a depth of live times the 1/e attenuation, depth for at least one characteristic x-ray signal of the analyte as attenuated by water. The method further comprises the step of removing at least a of portion of the analyte from the cells. The method also comprises the steps of exciting at least a portion of the analyte that has been removed from the cells with an x-ray beam; and using an x-ray detector to measure x-ray fluorescence.
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