Method for quantification of small RNA species

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United States of America Patent

PATENT NO 9096895
APP PUB NO 20120071332A1
SERIAL NO

13147562

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Abstract

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The present invention relates to a method for amplification and quantification of microRNA molecules using quantitative reverse transcription polymerase chain reaction (qRT-PCR) technology. The method comprise the steps of (a) producing cDNA molecules complementary' to microRNAs in a sample using polyadenylation and primer extension by reverse transcription, and (b) amplification and quantification of the cDNAs by qPCR using microRNA specific primer sets of forward and reverse primers containing LNA monomers.

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Patent Owner(s)

Patent OwnerAddress
QIAGEN GMBH40724 HILDEN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Busk, Peter Kamp Soeborg, DK 2 8

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