Apparatuses and compositions for cryopreservation of cellular monolayers
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United States of America Patent
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Sep 8, 2015
Grant Date -
Feb 16, 2012
app pub date -
Apr 29, 2010
filing date -
Apr 29, 2009
priority date (Note) -
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Abstract
Provided are apparatuses for cryopreserving cells which include a vessel comprising a biocompatible substrate, wherein the vessel further comprises an interior and an exterior, and a mechanical ice nucleating device disposed in or on the vessel interior for initiating ice crystal formation. Also provided are kits comprising one or more apparatuses for cryopreserving cells and a biopreservation medium. Further provided are compositions comprising a vessel for holding cells, a mechanical ice nucleating device, a biopreservation medium, and cells disposed in the vessel. The apparatuses, kits, and compositions of the invention can optionally include an insulating material which is disposed on all or a portion of the vessel.
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