Enzyme-based fed-batch technique in liquid cultures
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United States of America Patent
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Sep 8, 2015
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Mar 4, 2010
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Mar 5, 2009
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Abstract
The present invention is generally in the field of continuous and high-cell-density cultivation in laboratory- or large-scale liquid shaken cultures. More particularly it relates to a method of enzyme-based fed-batch (EnBase) for liquid microbial prokaryotic or eukaryotic cell cultivation having the possibility to manipulate the growth rate of the cultured organisms by a controlled enzymatic release of the growth-limiting substrate-monomer from substrate-polymers or substrate-oligomers.
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