Methods for performing direct enzymatic reactions involving nucleic acid molecules
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United States of America Patent
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Sep 8, 2015
Grant Date -
Jun 18, 2009
app pub date -
Feb 8, 2006
filing date -
Feb 28, 2005
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Abstract
Methods for performing a direct enzymatic reaction involving a nucleic acid molecule include performing the enzymatic reaction directly using a biological specimen in a reaction mixture containing a zwitterionic buffer and/or a non-reducing carbohydrate to prevent the biological specimen from inhibiting the enzymatic reaction, in which a nucleic acid molecule present in the biological specimen is not purified before the enzymatic reaction, and obtaining a product from the enzymatic reaction.
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