Methods for culture and production of single cell populations of human embryonic stem cells
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United States of America Patent
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Oct 13, 2015
Grant Date -
Feb 9, 2012
app pub date -
Jun 20, 2011
filing date -
May 25, 2006
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Abstract
We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.
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