Method for screening and quantifying isoprene biosynthesis enzyme activity
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United States of America Patent
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Nov 3, 2015
Grant Date -
Aug 21, 2014
app pub date -
Feb 20, 2013
filing date -
Feb 20, 2013
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Abstract
A method of performing high-throughput screening of various enzymatic activities with high sensitivity using artificial genetic circuits is provided. Particularly, the invention is screening and quantifying the activity of isoprene biosynthesis enzymes using an artificial genetic circuit capable of sensing isoprene. The artificial genetic circuit comprises an isoprene-sensing transcriptional regulator which recognizes isoprene, at least one reporter gene, a isoprene-sensing transcriptional regulator binding region and promoters for genes encoding isoprene-sensing transcriptional regulators and reporter proteins.
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