System and method for measuring narrow and wide angle light scatter on a high-speed cell sorting device
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United States of America Patent
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Nov 3, 2015
Grant Date -
Jul 4, 2013
app pub date -
Sep 20, 2012
filing date -
Jan 4, 2012
priority date (Note) -
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Abstract
The various embodiments disclosed herein utilize multiple lasers that have different wavelengths and a single detection path. The lasers are mounted orthogonal to one another so that one laser will provide a forward angle light scatter (FALS) signal in the detection path, and one laser will provide a side scatter signal in the detection path (i.e., the single detection optics are approximately in-line with the FALS laser and approximately orthogonal to the side scatter laser). The single detector path spectrally separates the forward and side scatter signals prior to applying them to their respective detectors for measurement.
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