Fabrication of nanopipette arrays for biosensing
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United States of America Patent
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Nov 10, 2015
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May 25, 2012
filing date -
May 25, 2012
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Abstract
Method for providing a nanopipette array for biosensing applications. A thin substrate of anodizable metal (“AN-metal,” such as Al, Mg, Zn, Ti, Ta and/or Nb) is anodized at temperature T=20-200° C., chemical bath pH=4-6 and electrical potential 1-300 Volts, to produce an array of anodized nanopipette channels, having diameters 10-50 nm, with oxidized channel surfaces of thickness 5-20 nm. A portion of exposed non-oxidized AN-metal between adjacent nanopipette channels, of length 1-5 μm, is etched away, exposing inner and outer surfaces of a nanopipette channel. A probe molecule, is deposited on one or both surfaces to provide biosensing capability for K(≧1) target molecules. Target molecule presence, in an above-threshold concentration, in a fluid passed through or adjacent to a nanopipette channel, produces characteristic detection signals associated with the probe molecule site.
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