In situ hybridization method and buffer
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United States of America Patent
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Dec 15, 2015
Grant Date -
Jun 27, 2013
app pub date -
Apr 28, 2011
filing date -
Apr 30, 2010
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Abstract
An improved method of in situ hybridization which relies on an improved formulation of the in situ hybridization buffer is described. In at least some formulations the buffer are non-toxic. The combination of Locked Nucleic Acid (LNA) comprising ISH probes and the improved ISH buffer are useful for detection of small non-coding RNA as well as in the manufacturing of ISH kits directed to the detection of such small non-coding RNA. Further disclosed is a method of semi-quantitative ISH and demonstration of the semi-quantitative ISHs diagnostic potential.
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