Method for amplification of nucleotide sequence

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United States of America Patent

PATENT NO 9222129
APP PUB NO 20090291852A1
SERIAL NO

11884084

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Abstract

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A method for amplification of a nucleotide sequence characterized by performing PCR amplification using mRNA isolated from a biological sample as a template and using a first primer comprising a poly(T) sequence and an additional nucleotide sequence X thereto and a second primer comprising a poly(T) sequence and an additional nucleotide sequence Y thereto, provided that the nucleotide sequences X and Y in the first and second primers, respectively, have different sequences from each other.

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Patent Owner(s)

Patent OwnerAddress
RIKEN2-1 HIROSAWA WAKO-SHI SAITAMA 351-0198

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kurimoto, Kazuki Hyogo, JP 1 2
Ohinata, Yasuhide Hyogo, JP 1 2
Saitou, Mitinori Hyogo, JP 15 15
Yabuta, Yukihiro Hyogo, JP 2 2

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