Single-molecule PCR on microparticles in water-in-oil emulsions

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United States of America Patent

PATENT NO 9260751
APP PUB NO 20150119287A1
SERIAL NO

14261837

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Abstract

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Modulation of the viscosity of the oil phase of a microemulsion used for amplification of DNA on a bead increases the homogeneity of product beads and the amount of amplified DNA per bead. Moreover the number of separate microemulsion populations that can be formed in parallel is increased using multi-well plates and mixer mill disrupter machines designed to lyse biological samples.

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Patent Owner(s)

  • THE JOHNS HOPKINS UNIVERSITY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Diehl, Frank Schortens, DE 18 235
Kinzler, Kenneth W Baltimore, US 281 4771
Vogelstein, Bert Baltimore, US 323 6299

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