Method for preparing quantitative video-microscopy and associated system
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United States of America Patent
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Mar 1, 2016
Grant Date -
Oct 18, 2012
app pub date -
Apr 11, 2012
filing date -
Apr 12, 2011
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Abstract
Embodiments of the present invention are directed to a method for calibrating an imaging system for analyzing a plurality of molecular species in a sample. According to one embodiment, the method comprises acquiring a plurality of images of the sample with an image acquisition device at a plurality of different wavelengths, comparing a region of interest associated with at least one of the images acquired at one respective wavelength to a region of interest associated with at least one of the images acquired at a different wavelength, and aligning the plurality of images such that the region of interest associated with at least one of the images acquired at one respective wavelength corresponds to the region of interest associated with the at least one of the images acquired at a different wavelength.
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