Amplicon melting analysis with saturation dyes

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United States of America Patent

PATENT NO 9290663
APP PUB NO 20120231522A1
SERIAL NO

13415240

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Abstract

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Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

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Patent Owner(s)

  • BIOFIRE DEFENSE, LLC;UNIVERSITY OF UTAH

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Dujols, Virginie Sandy, US 9 102
Reed, Gudrun Salt Lake City, US 6 54
Wittwer, Carl T Salt Lake City, US 58 2387
Zhou, Luming Salt Lake City, US 30 278

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