Preparation of an esterase
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United States of America Patent
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Apr 12, 2016
Grant Date -
Jul 21, 2011
app pub date -
Jul 4, 2008
filing date -
Jul 4, 2007
priority date (Note) -
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Abstract
The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.
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