Method for mass production of high-purity oligonucleotides
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United States of America Patent
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May 3, 2016
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app pub date -
Jun 15, 2012
filing date -
Oct 27, 2011
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Abstract
Provided is a method of mass-producing high-purity nucleotides including providing a sequencing substrate having a clonal library of oligonucleotides on a solid support, sequencing the clonal library, obtaining measured location data of the solid support on the sequencing substrate, mapping pixel data of a signal generated from the solid support given as a result of the sequencing with the measured location data, extracting the solid support having a desired base sequence from the sequencing substrate using the mapping result, and amplifying an oligonucleotide on the extracted solid support to replicate on a large scale.
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