Detection of target nucleic acid using a target hybridization and detection primer
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United States of America Patent
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May 3, 2016
Grant Date -
Aug 30, 2012
app pub date -
Nov 28, 2009
filing date -
Nov 7, 2009
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Abstract
The present invention relates to the detection of a target nucleic acid sequence using a target hybridization and detection primer (THD primer). The present invention allows for both a target amplification and a signal amplification by introducing a label into a primer used in PCR reactions, ensuring a real-time target detection by PCR reaction by no use of complicated oligonucleotides. The present invention could completely be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. This feature makes it possible that the present invention exhibits excellent real-time target detection in multiplex manner.
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