Method for high-throughput AFLP-based polymorphism detection

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United States of America Patent

PATENT NO 9334536
SERIAL NO

14274591

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Abstract

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The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.

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Patent Owner(s)

  • KEYGENE N.V.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Sørensen, Anker Preben Renkum, NL 12 936
Van, Eijk Michael Josephus Theresia Herpen, NL 73 4322
Van, Schriek Marco Gerardus Maria Bennekom, NL 13 829

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