Method for cultivation of genetically-modified plant

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United States of America Patent

PATENT NO 9353379
APP PUB NO 20120054923A1
SERIAL NO

13147436

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Abstract

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The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium,

    wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, andwherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.

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Patent Owner(s)

Patent OwnerAddress
NIPPON PAPER INDUSTRIES CO LTDTOKYO 114-0002

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kasahara, Saori Tokyo, JP 6 1
Shimada, Teruhisa Tokyo, JP 9 123
Sugita, Koichi Tokyo, JP 19 263
Wasai, Masafumi Tokyo, JP 6 5

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