Linear amplification of short nucleic acids

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United States of America Patent

PATENT NO 9394557
APP PUB NO 20140186893A1
SERIAL NO

14137786

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Abstract

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The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro RNAs by using temperature cycling during a reverse transcription reaction. The cycling can comprise at least 20 cycles of an annealing temperature segment of 10° C.-30° C., and a denaturation temperature segment of 35° C.-60° C. In some embodiments, the temperature cycled reaction can comprise an osmolyte.

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Patent Owner(s)

  • APPLIED BIOSYSTEMS, LLC

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bloch, Will White Salmon, US 32 1805

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