3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
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United States of America Patent
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Jul 26, 2016
Grant Date -
Jun 25, 2015
app pub date -
Oct 1, 2014
filing date -
Sep 13, 2011
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Abstract
The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
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