Method for purification of antibody using porous membrane having amino group and alkyl group both bound to graft chain immobilized on porous substrate
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United States of America Patent
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Sep 13, 2016
Grant Date -
May 17, 2012
app pub date -
Jun 29, 2010
filing date -
Jul 3, 2009
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Abstract
A method is disclosed for purifying an antibody monomer, comprising providing a porous membrane comprising a hydrophobic porous substrate, a hydrophilic molecular chain of a different material from that of the porous substrate, immobilized on the surface of pores of the porous substrate, and a side chain of the molecular chain, containing a nitrogen atom to which one to three alkyl groups each having two or three carbon atoms are bonded; passing an antibody solution containing antibody aggregates of dimers or higher-order multimers through the porous membrane to adsorb the antibody aggregates to the porous membrane; and recovering the purified antibody monomer in the passed solution.
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