Methods for multiplexing amplification reactions

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United States of America Patent

PATENT NO 9481907
APP PUB NO 20140329247A1
SERIAL NO

14302980

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A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.

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Patent Owner(s)

  • APPLIED BIOSYSTEMS, LLC

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Best, Elaine Ft. Collins, US 9 139
Gerdes, John C Denver, US 21 895
Marmaro, Jeffrey M Aurora, US 10 219

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