Methods and devices for multiplexed microarray microfluidic analysis of biomolecules

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United States of America Patent

PATENT NO 9481945
APP PUB NO 20130053273A1
SERIAL NO

13597362

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Abstract

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Rapid and specific detection of biological cells and biomolecules is important to biological assays across diverse fields including genomics, proteomics, diagnoses, and pathological studies. Microarrays and microfluidics increasingly dominate such detection techniques due to the ability to perform significant numbers of tests with limited sample volumes. A snap chip assembly is provided for the transfer of a microarray of reagents within semi-spherical liquid droplets on a transfer chip to a target assay microarray on an assay chip following assembly of the two chips and physical contact of the droplets with the target array. Reagents in nanoliter quantities are spotted on both chips and selectively transferred as liquid droplets between transfer chip and assay chip within the contact areas. Using the snap chip structure the inventors performed immunoassays with colocalization of capture and detection antibodies with 10 targets and bead-in-gel droplet microarrays with 9 targets in the low pg/ml regime.

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Patent Owner(s)

Patent OwnerAddress
THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING / MCGILL UNIVERSITYMONTRÉAL QUÉBEC H3A 0G4

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Juncker, David Verdun, CA 17 124
Li, Huiyan Montreal, CA 2 2

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