Combinatoric encoding methods for microarrays
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United States of America Patent
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Nov 8, 2016
Grant Date -
Jul 2, 2015
app pub date -
Jul 19, 2013
filing date -
Jul 20, 2012
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Abstract
A method of encoding a microarray includes depositing a first batch of particles on a substrate. The first batch of particles comprises a mixture of at least two sub-batches of the particles. Each of the sub-batches is capable of binding to a different, specific target analyte. Information necessary for decoding the microarray is provided prior to or during the depositing and includes a unique particle number ratio of the sub-batches. If more than one batch of particles is deposited, an image can be taken of the first batch prior to depositing subsequent batches to provide information about the position of the particles in the first batch of particles. These depositing and imaging steps can be sequentially repeated for the subsequent batches. Such a microarray having multiple batches can be decoded using the information about the position of the particles and the known particle number ratios of the sub-batches.
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