Systems and methods for feedstock quality assessment

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United States of America Patent

PATENT NO 9487817
APP PUB NO 20130337483A1
SERIAL NO

13993056

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Abstract

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Assessing quality of feedstock is provided and may be useful for determining quality of feed (such as corn kernels). The assessment determines endogenous enzyme activity within the feedstock, which correlates with total ethanol yields in raw starch hydrolysis (non-cooked) systems. In some embodiments, a sample containing flour (ground feedstock) is provided. In some cases, flour is diluted in water and buffered with a phosphate buffered solution. The buffered flour solution is contacted with a molecule, or fluorescent dye, such as fluorescein diacetate and/or difluorofluorescein that is altered by enzymatic (esterase) activity in a detectable fashion. Enzyme activity may be facilitated using an incubator. The detectible alteration may be measured using a fluorometer. In some embodiments, two incubations and fluorescence measurements can be performed. By subtracting the difference between the measured fluorescence, a fluorescence index may be found, which correlates with the endogenous enzyme activity in the feedstock sample.

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Patent Owner(s)

Patent OwnerAddress
POET RESEARCH INC4615 N LEWIS AVENUE SIOUX FALLS SD 57104

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Lewis, Stephen M Sioux Falls, US 24 274
Narendranath, Neelakantam V Sioux Falls, US 20 135

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