Method of detecting surrogate markers in a serum sample
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United States of America Patent
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Nov 29, 2016
Grant Date -
May 30, 2013
app pub date -
Jul 12, 2011
filing date -
Jul 15, 2010
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Abstract
The present invention relates to a method of detecting an active tuberculosis infection in a serum sample from an individual. More specifically, the disclosed method uses a single chain variable fragment from a monoclonal immunoglobulin specific for mycolic acids of mycobacterial origin to detect surrogate markers, the presence of which indicate the individual has an active tuberculosis infection. Markers useful for practicing the disclosed method can be serum mycolic acid antigen, serum anti-mycolic acid antibodies or both. In a preferred embodiment, the method is practiced using a lateral flow assay format.
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