Method for measuring analyte transport across cell membranes using X-ray fluorescence
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United States of America Patent
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Nov 29, 2016
Grant Date -
Jul 16, 2015
app pub date -
Mar 26, 2015
filing date -
Jul 1, 2008
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Abstract
The present invention includes a method and apparatus for measuring the transport of an analyte through a cell membrane. One or more cells expressing a plurality of ion channels are provided. These cells are loaded with the analyte so that the cells contain at least 10 picograms of the analyte within a volume defined by the area of an x-ray excitation beam and a depth of five times the 1/e attenuation depth for at least one characteristic x-ray signal of the analyte as attenuated by water. The unloaded analyte is removed and the amount of the analyte in the cells is measured with x-ray fluorescence.
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